8 Questions 12 Answers 0 Followers
Questions related from Mohammad Vatanparast
We bought this kit (MEGAscript RNAi Kit) and following the instructions, we kept it at -20. But one of the micro tube that contains 10x binding buffer was cracked and we have lost buffer. It was...
05 May 2016 9,539 2 View
I am rearing Helicoverpa armigera at 25°C and 70% humidity. the insects eggs is not hatched. we have about 1000 eggs but after 10 days, we do not have any larvae.
12 December 2015 3,697 19 View
I want to know what is difference between "Not determined" or "ND" with "Negative" or "-" in results in microbiology research. I studied a paper that a series of microorganisms were cultured on...
07 July 2015 5,941 1 View
If the concentration of ethidium bromide is very high, what will have effects on visibility of bonds production on agarose gel. What is the best alternative for ethidium bromide?
03 March 2015 2,657 3 View
I am looking for the nucleotide sequence of a particular gene in NCBI. But results obtained in large numbers and as predicted. Are sequences predicted to be trusted? How might one obtain these...
11 November 2014 7,861 4 View
We have a column for gel filtration chromatography (120 cm*1cm) that we will use for enzyme purification. We want to load it by Sephadex G-75. What height (cm) of column packed with sephadex is...
10 October 2014 6,867 18 View
We want to purify our proteins. After dialyzing the protein we eluted them by concentration gradiant of NaCl in Ion exchange column. We need to know concentration of our fractions. For this, we...
04 April 2014 1,347 7 View
For proteases.
02 February 2014 7,556 3 View
