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I'm using Fractogel DMAE column. after each run I incubate column with NaCl 2M to bring down everything in column. After that we clean column with NaOH 1 N and always get a big peak in this phase...
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Yesterday I was harvesting some cells and made a mistake in diafiltraion phase ..suddenly my hollow fiber started to foul in this phase despite the flux was under the critical and the flow was as...
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