Mohammad K. Zim

2 Questions 1 Answers 0 Followers

Questions related from Mohammad K. Zim

What is the best lysis buffer for sf9 cells ?

I'm trying to isolate proteins from sf9's cytosol but I have a problem with cell lysis . Any idea for lysis buffer or lysis protocol ? PS: we do NOT want to centrifuge cells then lyse them .. we...

14 February 2016 1,390 2 View

How do I clean hollow fiber?

Yesterday I was harvesting some cells and made a mistake in diafiltraion phase ..suddenly my hollow fiber started to foul in this phase despite the flux was under the critical and the flow was as...

21 January 2016 2,943 3 View