Mohamed Kamal

5 Questions 10 Answers 0 Followers

Questions related from Mohamed Kamal

Could Biotinylated antibodies be visualized fluorescentely?

We are using the streptoavidin dynabeads system to magnetically eliminate immune cells from whole blood which are labeled with CD45 biotinylated antibodies. After the magnetic separation step,...

24 February 2022 9,757 3 View

Is there anyone expanding Circulating tumour cells in vitro?

I am planning to culture and expand circulating tumour cells in vitro. It would be very much appreciated if you share your experiences with me. Thanks Mohamed

28 January 2017 4,019 2 View

Could I do ADCC assay using NK cells isoalted by positive selection?

I am planing to isolate NK cells from mice and human blood to use in antigen dependent cytotoxicity assays (ADCC). I am worried that the positive selection of NK affects thier performance in this...

08 July 2016 4,368 2 View

Do I have to confirm the qPCR array (Stell Array, Lonza) data using specific qPCR assays before testing the protein expression of these genes?

I have used qPCR arrays to test the expression of 48b genes in two groups of cells. These arrays showed 10 genes which their expressions are significantly different between the groups. The next...

29 September 2013 9,077 2 View

Does anyone tried mammosphere assay for circulating tumour cells?

I am wondering if it is possible to do mammosphere assay for individual Circulating tumour cells. If someone has experience in this it would be much appreciated to share it with me. Thanks

01 January 1970 1,871 3 View