Mirko Pavicic

3 Questions 10 Answers 0 Followers

Questions related from Mirko Pavicic

Is there any other free option to choose qPCR reference genes than geNorm?

I was wondering if there is other option than geNorm to pick some reference genes for RQ qPCR than geNorm. My question arises from geNorm use to be free, now you have to paid for it. So, before to...

11 November 2013 1,091 1 View

How can I estimate statistical differences between calibrator and test conditions in Relative Quantification qPCR?

Dear colleagues: I'm trying to find out, how I can estimate significative up - regulated genes by relative quantification qPCR (RQ) using Pfaffl method. This method gives as results fold of...

08 August 2013 5,441 14 View

Which is the best choice to determinate qPCR amplification efficiency? cDNA, gDNA or plasmid serial dilutions?

Dear colleagues I'm working with qPCR relative quantification and I need to determine the efficiency of qPCR amplification. I know we can use dilution series from gDNA, cDNA or template inside a...

07 July 2013 8,512 17 View