Miriane de Oliveira

6 Questions 11 Answers 0 Followers

Questions related from Miriane de Oliveira

Does anyone recommend a kit that allows co-extraction of DNA and RNA from slides and effectively preserves microRNAs?

Hello everyone, At our lab, we currently perform manual co-extraction of DNA and RNA from FNA (fine-needle aspiration) slides using Trizol reagent and this protocol is effective in preserving...

10 June 2025 7,415 1 View

More effective and faster solvent than xylene for removing coverslips from cytology slides?

Hi everyone, In the diagnostic workflow of the test, we often need to reuse cytology slides, which requires removing the coverslip previously mounted over the sample. Currently, we use xylene, and...

11 March 2025 6,916 4 View

Does anyone have experience with Agilent NGS for mutation and fusion?

We are in discussions with Agilent to acquire a custom mutation and fusion panel. They introduced us to the pre-capture polling solution. I would like to know about the experience of those who...

15 May 2023 9,499 2 View

The whole transcriptome amplification mRNA from the FFPE samples posterioly cDNA synthesis, is necessary?

We will qPCR approximately 95 genes, verify gene expression profiles, FFPE mRNA. I would like to know if you think it is necessary to perform a "pre-amplification", previous cDNA synthesis, to...

06 February 2023 1,479 0 View

How can we explain the expression increased by a treatment of a certain protein, and the mRNA of that protein was decreased by that treatment?

We made the global transcriptome (global mRNA expression), by RNAseq, from human subcutaneous adipocytes. Subsequently we did the Western blot for some proteins that were determined from the...

22 February 2021 5,021 6 View

Equipment NGS to analyze mutations (oncology panel)

I would like your opinion on choosing a device to analyze mutations in a certain number of genes (20 to 30), I believe the sequencing of some 500 amplicons, of metastatic tumors (approximately 230...

01 January 1970 1,556 0 View