Mingming Sun

5 Questions 9 Answers 0 Followers

Questions related from Mingming Sun

in vitro phosphorylation with a ready phosphrylated protein?

I am trying to do in vitro phosphorylation for one intersting SR protein. I already have the recombined-protein purified from insects cells and with mass spectra to confirm its phosphrylation...

23 May 2018 7,457 5 View

In vitro phosphrylation without 32p?

As 32p looks kind of dangerous and complicated to apply the protocal, is there any alternative method for this kind in vitro phosphrylation? Thanks

05 April 2017 9,769 3 View

How to make typeII diabetes with 45% or 60% high fat diet?

Hi people, I am trying to make typeII diabetes model with 45% or 60% high-fat diet. Anyone has experence about this? I wonder if feed the rats with high fat diet since 2 month old, how long should...

23 March 2017 2,025 3 View

Is there any better way to sperate PCR products 258bp and 222 bp?

With PCR, it is supposed to get two bands of PCR products one is 258 bp and another is 222 bp. Anyhow, with 2% gel, only one bold band could be seen(>200 bp) and if run it longer, only smear...

25 January 2017 5,884 6 View

Why when performing IPGTT, the gluocose of blood after 15 min didn't increase to very high?

glucose (50%) was injected IP, then the glucose in blood was measured. The problem was, the glucose in bood of rats did not increase very fast and high. For example, the glucose in some rats was...

14 September 2016 9,607 10 View