Minakshi Gandhi

2 Questions 8 Answers 0 Followers

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Circulating serum microRNAs?

I am using miRNeasy isolation kit from Qiagen and using 100 micro liter serum as a starting volume. Centrifugation after chloroform addition gives only 2 phases after 15 mins, 4 deg and 16000 rpm...

05 May 2013 2,116 8 View

How to separate plasma from frozen blood?

Does anyone know how to separate plasma from frozen blood? I have tried centrifugation, ficoll hypaqu density centrifugation etc ..

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