Millie Lam

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Questions related from Millie Lam

Illumina HT12 v4 bead array: forgot to heat High Temp Wash Buffer overnight?

I am did hybridization of the Illumina HT12 v4 bead array today. I am supposed to heat the High Temp Wash Buffer to 55C overnight to wash the bead array the next day. However, I have forgotten...

05 April 2017 7,903 0 View

This question is with regards to neural differentiation - EBs are not attaching to laminin. Why?

I have been trying neuronal differentiation from human embryonic stem cells (hESCs; H9) via the hESC aggregate method according to http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2948208/ and...

07 December 2014 4,257 1 View

What is the staging of neuronal differentiation of human embryonic stem cells/pluripotent stem cells?

I am currently doing neuronal differentiation of human embryonic stem cells. I realized that it is hard to find papers that stage the neuronal differentiation process (usually they just describe...

18 November 2014 7,167 4 View

Has anyone managed to get neural rosettes from neuronal differentiation by the hESC aggregate method using Matrigel-coated plates?

I have been trying neuronal differentiation from human embryonic stem cells (hESCs; H9) via the hESC aggregate method from Zhang's lab (Methods Mol Biol. 2010;584:355-66. doi:...

08 November 2014 236 8 View

Why would one get a low fold induction upon doxycycline treatment?

Has anyone used the FUW-tetO system for doxycycline-induced transgene expression? I transduced my cells with trangenes (MOI=5 each) under the FUW-tetO, then treated with 1 ug/mL doxycycline for 5...

10 October 2014 2,083 7 View