Millie Lam

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Questions related from Millie Lam

What is the staging of neuronal differentiation of human embryonic stem cells/pluripotent stem cells?

I am currently doing neuronal differentiation of human embryonic stem cells. I realized that it is hard to find papers that stage the neuronal differentiation process (usually they just describe...

18 November 2014 7,163 4 View

Has anyone managed to get neural rosettes from neuronal differentiation by the hESC aggregate method using Matrigel-coated plates?

I have been trying neuronal differentiation from human embryonic stem cells (hESCs; H9) via the hESC aggregate method from Zhang's lab (Methods Mol Biol. 2010;584:355-66. doi:...

08 November 2014 231 8 View

Why would one get a low fold induction upon doxycycline treatment?

Has anyone used the FUW-tetO system for doxycycline-induced transgene expression? I transduced my cells with trangenes (MOI=5 each) under the FUW-tetO, then treated with 1 ug/mL doxycycline for 5...

10 October 2014 2,077 7 View