Why would one get a low fold induction upon doxycycline treatment?

Has anyone used the FUW-tetO system for doxycycline-induced transgene expression? I transduced my cells with trangenes (MOI=5 each) under the FUW-tetO, then treated with 1 ug/mL doxycycline for 5...

10 October 2014 2,070 7 View