Mike Watson

5 Questions 7 Answers 0 Followers

Questions related from Mike Watson

Can you help me select an antibody labeling kit?

Hello Everyone, I'm in need of recommendations to label primary monoclonal and polyclonal antibodies that i'm using to make a specific flow cytometry panel. Some of the markers are low expressed...

02 February 2019 4,062 3 View

Does an off target sequence mean my TaqMan Assay is invalid?

Hey Molecular Biology folks, I've got a question for you. I'm developing TaqMan assays on cell lysates. I have a bunch of probes that when I run them for 40 cycles in RT-qPCR I get nice signal...

10 October 2018 1,012 1 View

Does anyone titrate their secondary antibodies for intracellular flow?

Hey everyone, I'm working on creating a flow QC panel. I have a few sets of 3 intracellular and 3 surface markers that I know have worked well in the past for ICC and flow applications. My...

09 September 2018 1,317 1 View

My extracted RNA from cells has good A260/A280 purity but poor A260/230 purity. What can I do?

Hello All My Microbio Experts, I'm a total noobie in this field so go easy... I've extracted RNA from cells and the A280/A260 values look good (~2.0 for most samples) which means the RNA is...

08 August 2018 4,758 1 View

Why is my isotype staining so high relative to unstained but only with AF647-tagged antibody?

Hello Everyone, I have a flow cytometry question. I'm evaluating ES cells for percent positive amounts of two pluripotency markers: Oct4 and Tra-1-60. I'm staining cells post-fix and perm with the...

06 June 2018 8,649 11 View