11 Questions 17 Answers 0 Followers
Questions related from Mikael Niku
Which RNA extraction method would you recommend for RNA-Seq of snap-frozen fetal murine tissues? We will be doing Lexogen QuantSeq. Considering Qiagen RNeasy (good, but pretty expensive) or Thermo...
03 October 2019 9,611 3 View
Does anyone know a resource for data on organ sizes (mass, preferably also dimensions) for fetal mice at various stages of development?
15 March 2019 2,295 1 View
RNA extraction from laser microdissected FFPE tissues is markedly enhanced by sonication (paper below). However, I could only find a kit and protocol for Covaris sonicators. We only have a...
21 December 2018 3,017 1 View
We are happily extracting bacterial DNA using a bead beating protocol. Now we need to start processing swab samples containing fecal etc bacteria. If we put the entire swab into the bead beating,...
27 June 2016 439 4 View
I'm trying to visualize intestinal bacteria in paraffin sections. The problem is, the bacterial cells seem to shrink so much that no shapes are preserved, they are all just tiny roughly circular...
21 April 2015 7,062 12 View
Hello! What is the best histological stain or immunostaining marker to visualize bacteria in sections of animal tissues? Optimally, staining all (or most) bacteria but no eukaryotic cells, and...
27 October 2014 2,867 6 View
I'm having trouble with contamination of linearized plasmid with the intact circular plasmid. I have heavily overdigested the plasmid, gel purified, and digested with another restriction enzyme,...
26 November 2013 1,986 5 View
I'm using sodium butyrate in cell culture, but I'm slightly worried about the stability of my reagents. There is very conflicting information available regarding its stability in aqueous or...
11 March 2013 6,628 2 View
Hello! May I ask for your recommendations for tissue processing methods for laser microdissection and subsequent RNA isolation? I can think of at least the following protocols, each with...
13 February 2013 1,616 15 View
I have problems getting very weak fluorescence signal from my probe-based RT-qPCR assay. The Ct is not bad (less than 30, and I see a clear although weak band on gel), but the signal intensity is...
18 January 2013 1,829 3 View
We are planning to do RNA-Seq for RNA extracted from two types of samples: Routine snap-frozen mouse fetal tissues Laser microdissected tissue sections (FFPE sections and/or cryosections) We only...
01 January 1970 825 2 View
