Michael Mackillop

4 Questions 3 Answers 0 Followers

Questions related from Michael Mackillop

Nevermind, got it.?

whooooops. Figured it out

04 April 2019 5,782 3 View

Heating final cDNA library to elute from PAGE?

I am currently working on a small RNA sequencing experiment, and am having difficulties with the final step of library preparation. Several of the protocols I follow say that the final gel...

10 October 2018 363 2 View

Best extraction buffer/quantification options for isolating mitochondrial proteins?

I'm wondering if anyone has had great success with any particular type of buffer for extracting mitochondrial proteins from purified whole mitochondria. I'm relatively new to the protein game, and...

10 October 2016 7,792 1 View

Does anyone have a protocol for isolating IMM and OMM in fungus?

I've protocols for isolation of membrane bound proteins via treatment with ice cold sodium carbonate. If anyone could speak to the efficacy of this vs centrifugation, that would be swell.

10 October 2015 1,102 3 View