Michael Gines

3 Questions 9 Answers 0 Followers

Questions related from Michael Gines

Real-Time PCR: target threshold or fluorophore threshold?

I am using a biorad CFX machine to do real-time PCR. I am trying to validate candidates for a reference gene. In their program, the threshold can be calculated for each target (primer set) or by...

01 June 2016 6,137 3 View

In expression analysis, have you experienced PCR inhibitors from your cDNA kit?

I have repeatedly tested my primer efficiency values for several pairs and consistently get high numbers-- from 120-300%, with R^2 values at .99 I test six dilutions and have done this using...

20 July 2015 8,057 8 View

What do you suggest for high primer efficiencies?

I am doing real time PCR on 20 genes of interest on oat and barley tissues. I am constantly getting clustered curves around 24-26 cycles for my first three dilutions before the rest begin...

23 June 2015 9,417 9 View