Miao Li

4 Questions 3 Answers 0 Followers

Questions related from Miao Li

Why my target protein from co-IP samples reduced its molecular weight?

Hello everyone, hope you are doing well. I am doing the co-IP assay recently. My aim is analyse the interaction of protein A and protein B. Importantly, protein A can be glycosylation. I...

18 September 2016 2,004 3 View

Why NF-KB p65 translocation into the nuclei of RAW264.7 occured even when untreated?

Hello everyone, I am doing the immunofluorescence assay. And I want to see the NF-KB p65 translocate from cytoplasma to nuclear. But in my MOCK treated group, p65 still in the nuclei and very...

25 December 2015 623 6 View

Why does my CFSE assay look horribe?

Hello everyone, I am trying to improve my CFSE assay of culturing mouse CD4 T cells. I separated mouse whole CD4 T cells with beads, and culture in 96-wells. I used CD3/CD28 to stimulated the...

05 November 2015 7,129 4 View

Can someone explain to me the MLR (MHC alloantigens in mixed leukocyte reactions) test?

Hello, Can someone explain me the MLR(MHC alloantigens in mixed leukocyte reactions) test? I know the MLR means APC co-culture with heterogous CD4+ T cells, and can promote T cells proliferation....

29 November 2014 6,047 6 View