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Questions related from Mia Blum
Hello, I want to do EMSA with native PAGE to check protein-dna interactions. The PIs of my proteins are between 8.1-8.5. I know that the pH of my buffer must be higher, so that the net charge is...
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Hello, I don't understand the difference in what you can see after running your DNA on an urea or an alkaline gel. I know how alkaline works: high pH, hydrogen bonds are prevented, as a result...
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Hello, I expressed a protein with a his tag (65 kDa) and purified it by Ni-NTA affinity chromatography. The amount of protein that I got was huge, so I didn't wonder about the aggregation in the...
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Hello everyone, I need genomic E. coli DNA as a template for later PCR. I have a protocol how to isolate genomic DNA and it says I have to prepare an overnight culture of XL1 blue cells. We don't...
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