Metty Ariani

5 Questions 10 Answers 0 Followers

Questions related from Metty Ariani

Can we perform melt curve analysis with TaqMan probe?

I've searched it before and it says that taqMan probe couldnt be used for melt curve analysis. However, what about TaqMan probe with MGB? I found in some article that MGB probe could be used for...

31 May 2024 836 0 View

Why is my specific primer/probe detects some ct in negative control?

I have some set of specific primer/probe. I've checked in on BLAST and gel electrophoresis and it was specific. However, when I run it in qPCR there are some ct value detected in negative control...

28 April 2024 1,331 2 View

Is ct value > 30 in negative control acceptable?

Hi there! Im running a qpcr right now. For negative control, I had two samples that is contain DNA template but supposedly undetected by my primer (I've checked this on primer BLAST) and NTC....

04 April 2024 4,154 4 View

Does qPCR efficiency should be the same/ similiar each time we run qPCR?

I have this primer which is targetted for certain gene. Since I'm using the relative standard curve methods, plate that is available in my lab is only 48 wells. I have a quite large of samples...

04 April 2024 8,102 0 View

What kind of samples that could be used as a standard curve?

Right now, I'm doing an experiment by using spiked sample with porcine DNA. I wanna know what is the LoD from the primer that I used. For sample, I used 10% spiked, 1% spiked, and 0.1% spiked....

03 April 2024 1,401 10 View