Metty Ariani

4 Questions 10 Answers 0 Followers

Questions related from Metty Ariani

Why is my specific primer/probe detects some ct in negative control?

I have some set of specific primer/probe. I've checked in on BLAST and gel electrophoresis and it was specific. However, when I run it in qPCR there are some ct value detected in negative control...

28 April 2024 1,184 2 View

Is ct value > 30 in negative control acceptable?

Hi there! Im running a qpcr right now. For negative control, I had two samples that is contain DNA template but supposedly undetected by my primer (I've checked this on primer BLAST) and NTC....

04 April 2024 4,012 4 View

Does qPCR efficiency should be the same/ similiar each time we run qPCR?

I have this primer which is targetted for certain gene. Since I'm using the relative standard curve methods, plate that is available in my lab is only 48 wells. I have a quite large of samples...

04 April 2024 8,049 0 View

What kind of samples that could be used as a standard curve?

Right now, I'm doing an experiment by using spiked sample with porcine DNA. I wanna know what is the LoD from the primer that I used. For sample, I used 10% spiked, 1% spiked, and 0.1% spiked....

03 April 2024 1,361 10 View