7 Questions 12 Answers 0 Followers
Questions related from Mert Burak Öztürk
Dear all, When I run my yeast total protein lysate extracted after 10 minute-treatment with my compound, I observe a global up regulation in proteins, even my loading control protein. I suspect...
04 January 2021 1,527 8 View
Hi all, I am not sure whether or not this question was asked. I wonder can I induce expression of my protein with tetracycline or doxycyxline while seeding cells instead of one day after seeding...
06 November 2020 2,563 4 View
Hi, I am trying to transfect 293T cells using 2M CaCl2 and 2X HBS (50 mM HEPES, pH 7.07, 10 mM KCl, 12 mM dextrose, 280 mM NaCl, 1.5 mM Na2PO4) However, some plates, still have particles (probably...
24 March 2018 5,936 7 View
Hi, Is there anyone who has done siRNA Transfection in COLO205, SW620, HT29 especially using either RNAiMAX or Lipofectamine3000? Besides do you have any experience to transfect/transduce plasmid...
05 December 2017 2,455 3 View
Hi Everyone, May i ask who has normalized his/her IP results for interaction? especially considering equal over-expression, equal loading into a gel and equal pull-down across samples? Thanks,
08 June 2016 5,512 3 View
Hi, I am having trouble with cells overexpressed mutant IDH enzyme. Problem is that it does not work. In other words, I chose some genes as a positive control from other papers and evaluated their...
03 March 2016 6,422 3 View
RWPE-1 is a prostate epithelial cell line. I use Keratinocyte-SFM with recombinant human Epidermal Growth Factor (rhEGF) and Bovine Pituitary Extract (BPE). It doesn' t include Pen./Strep....
16 July 2014 698 10 View
