Mercy Chen

5 Questions 1 Answers 0 Followers

Questions related from Mercy Chen

How to prepare an internal control using the clone pathogen

Hi, From the 10pg concentration I want to dilute this to the ff concentration:1. 0.083 fg2. 0.167fg3. 0.33 fg this experiment is to validate the membrane dot (hybridisation) is sensitive enough to...

13 July 2017 2,514 1 View

How to select the peaks for bio marker detection using MALDI-TOF clip to tool software?

Im having doubts on myself of just adding and removing some peaks for my research regarding the bio marker detection. (VRE versus VSE) From the clinprotool software on Maldi-Tof, There's artefacts...

01 July 2017 7,249 0 View

What membrane (silica membrane) should I use for the extraction?

For improvisation of the extraction procedure, we would want to skip the step of washing procedure during the extraction. Are there any literature that cite about the spin column membrane which...

12 June 2017 3,015 2 View

Why does the slope looks like in vii7 (realtime)?

The test is for detecting the direct qualitative for Chlamydia trachomatis and Neisseria gonorrhoea. We use FAM (CT), JOE (NG) reporter and CY5 (beta for control). Results are reportable after we...

08 June 2017 9,589 2 View

Could anyone know how to troubleshoot the fragment analysis of trisomy? I have noticed that the peak's height are very low (10-20)

We use panel A (D13S258, D13S37, D13S63, D13S796, D18S1002, D18S391) and B marker (D18S851, D18SS535, D21S11, D21S1411, D21S1435, D21S1446). In addition, we do back up marker if marker is missing...

08 June 2017 5,735 0 View