Melissa Sander

4 Questions 4 Answers 0 Followers

Questions related from Melissa Sander

QPCR and probe with mismatched bases, how many allowed?

Can't find anything definitive in the published literature....wondering if anyone has firsthand experience with qPCR and probe mismatches. How many mismatched bases can be tolerated? I assume...

30 May 2022 1,453 0 View

Does anyone know of an alignment tool for dsDNA sense/antisense?

I'm having trouble wrapping my brain around an assay I'm designing and would LOVE to find an online tool to show an alignment of my sense and antisense strands. I've found plenty of tools to give...

21 March 2022 9,113 3 View

How to use BLAST to search for potential amplicons from a primer pair....?

I want to search for any and all amplicons that could be produced from my primer pairs. My template will be DNA from human stool samples. My primers are intended to amplify specific microbes, but...

25 February 2022 3,291 4 View

QPCR melt curve question!

I'm getting 2 very messy peaks on my qPCR melt curve. The melt temp ranges from 82-89, so not a massive difference, but definitely enough so that it's not a nice clean single peak. Does anyone...

01 January 1970 1,068 0 View