Melissa Sander

2 Questions 2 Answers 0 Followers

Questions related from Melissa Sander

QPCR and probe with mismatched bases, how many allowed?

Can't find anything definitive in the published literature....wondering if anyone has firsthand experience with qPCR and probe mismatches. How many mismatched bases can be tolerated? I assume...

30 May 2022 1,389 0 View

Does anyone know of an alignment tool for dsDNA sense/antisense?

I'm having trouble wrapping my brain around an assay I'm designing and would LOVE to find an online tool to show an alignment of my sense and antisense strands. I've found plenty of tools to give...

21 March 2022 9,107 3 View