9 Questions 23 Answers 0 Followers
Questions related from Mehrdad Rabiei
Hi all, Hope you are safe and sound. I have a question for you regarding nucleotide number positions of the two primers (R and F) on the PCR product. I example F-926 /R-1392 universal 16s primer...
01 January 1970 1,602 13 View
I have two forward and reverse reads from HiSeq Illumina platform. The PCR amplicon was 480 bp, 2*240. In merging, how will they have overlapping while they have no common bases? I am just...
01 January 1970 6,803 5 View
For the first time, I am going to extract plasmid. I would like to know whether gDNA would be extracted during the plasmid extracting? Similarly, is the plasmid extracted when gDNA getting to be...
01 January 1970 6,262 3 View
please reply to me regarding this question. Best regards,
01 January 1970 324 4 View
I got confused between two negative controls: NTC (No Template Control) and NC (Negative Control). Am I right? NTC: No DNA or nuclease-free water as a template? NC: Added nuclease-free water as...
01 January 1970 5,796 2 View
Conversion of peak area' values of HPLC to concentration!
01 January 1970 4,712 14 View
Hi everybody, Do you have any idea how the databases are made? In other words, what processes they take to be generated? By the way, how they collect the new sequences and their taxonomies? And...
01 January 1970 3,277 0 View
Hi dear researchers, While two organisms sharing ≥97% identical 16S rRNA genes may or may not belong to the same species, so why many researchers using 16S rRNA gene for their studies? For...
01 January 1970 2,733 2 View
Hi all, I analysed mcrA gene reads by Qiime2. I extracted taxonomy and Sequence references from NCBI website. The result I got is unsatisfied, in my view, because many of taxa are uncultured and...
01 January 1970 1,102 0 View