8 Questions 28 Answers 0 Followers
Questions related from Meaghan Roy-O'Reilly
Our lab is currently writing a manuscript on differential gene expression in RNA-sequencing data. As we're not a sequencing-based lab, we were hoping to find some free pathway analysis software....
11 November 2017 1,285 3 View
In our lab, we generally use 3 rounds of 10 minute, 4* incubation with tris ammonium chloride (Stem Cell Technologies) for red blood cell lysis of murine blood. However, neutrophil counts by...
11 November 2017 6,440 5 View
We're studying differential miRNA expression 24 hours after injury in human clinical samples. What are the advantages/disadvantages of using whole blood (cellular + extracellular) instead of serum...
04 April 2015 8,087 5 View
I'm currently conducting research with human subjects and would like to analyze human neutrophils by Western Blot. We get 1-2 patients a week -- would it be possible to flash freeze the...
01 January 2015 1,813 4 View
I'm currently developing a human flow panel for peripheral blood cells. The lymphocyte and monocyte sub-populations (CD4, CD8, CD20, CD14, CD16) look as expected, but although we are getting a...
10 October 2014 3,825 7 View
We're conducting flow cytometry on human blood that has been separated into two fractions using FiColl -- a buffy coat layer containing mostly PBMCs and a red layer enriched in granulocytes. While...
10 October 2014 9,339 6 View
Hello -- I'm looking to develop a flow panel for human PBMCs and PMNs that utilizes fresh samples. We fix first and then stain with antibodies. If my cells are fixed and sitting at 4*C, how many...
09 September 2014 2,117 19 View
I've seen a few different recipes for staining/washing buffers used in flow cytometry of human PBMCs and neutrophils, including PBS with 2% BSA and PBS with 0.5% BSA and 0.03% NaN3. We fix our...
09 September 2014 6,091 10 View
