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Our lab is currently writing a manuscript on differential gene expression in RNA-sequencing data. As we're not a sequencing-based lab, we were hoping to find some free pathway analysis software....
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In our lab, we generally use 3 rounds of 10 minute, 4* incubation with tris ammonium chloride (Stem Cell Technologies) for red blood cell lysis of murine blood. However, neutrophil counts by...
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I'm currently conducting research with human subjects and would like to analyze human neutrophils by Western Blot. We get 1-2 patients a week -- would it be possible to flash freeze the...
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I'm currently developing a human flow panel for peripheral blood cells. The lymphocyte and monocyte sub-populations (CD4, CD8, CD20, CD14, CD16) look as expected, but although we are getting a...
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We're conducting flow cytometry on human blood that has been separated into two fractions using FiColl -- a buffy coat layer containing mostly PBMCs and a red layer enriched in granulocytes. While...
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Hello -- I'm looking to develop a flow panel for human PBMCs and PMNs that utilizes fresh samples. We fix first and then stain with antibodies. If my cells are fixed and sitting at 4*C, how many...
09 September 2014 2,166 19 View
I've seen a few different recipes for staining/washing buffers used in flow cytometry of human PBMCs and neutrophils, including PBS with 2% BSA and PBS with 0.5% BSA and 0.03% NaN3. We fix our...
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