Md Saiful Islam

8 Questions 23 Answers 0 Followers

Questions related from Md Saiful Islam

What is/are the necessary pretreatment steps for ICP-OES analysis of Aluminum containing environmental sample?

For the usual environmental sample containing metals, we do pretreatment with HNO3 and H2O2. Adding those solutions and heating, reducing the volume, removing organic content etc. Is there any...

11 January 2024 2,553 2 View

What are the major constraints of the use of Ozonated water as disinfectants?

As I know, ozone has comparatively less impacts than chlorinated water or bleaching agent, why people are not so willing to use the ozone water. Is it due to the market barrier or something...

07 September 2017 3,759 18 View

Is it necessary to digest the livers from embryos in collagenase solution for hepatocytes preparation in order to carry out EROD measurement?

I found one literature that livers from embryos were pooled and digested for 45 min in 75 mL of a 0.5 mg/mL collagenase (Sigma) solution. Actually what is the purpose of adding collagenase...

17 May 2016 6,534 1 View

How to prepare the real samples for the determination of microcystin-LR in Protein phosphatase inhibition assay?

I am currently doing PP2A inhibition assay for determining Microcystin-LR. But in case of real environmental water, how to prepare the samples?

17 March 2016 357 1 View

Which technique is best for measuring EROD activity?

Usually in EROD assay, resorufin production from EROD by enzymatic reaction is measured by fluorimetric method. Is there any scope to implement Spectroelectrochemistry in EROD assay? Will it...

02 March 2016 3,674 0 View

Is it necessary to measure the protein concentrations during EROD assay with fish liver microsome?

I found in some literature that they measure the total protein concentration according to the Bradford method. But I dont know why it is necessary. Thanks in advance.

01 January 1970 1,661 2 View

Is there any fixed range for Michaelis-Menten constant Km ?

I am now checking the PAPP substrate with PP2A at pH 8.4.

01 January 1970 2,764 3 View

In EROD assay is it mandatory to isolate the microsomal part for fluorometric determination ?

What kind of problem may face without isolation ? Usually after homogenization the liver, the microsomal part is separated and used for EROD assay?

01 January 1970 6,544 3 View