Md. Manirujjaman

5 Questions 12 Answers 0 Followers

Questions related from Md. Manirujjaman

How bacterial genome can be replaced with genetically modified plasmid?

After transformation how the bacterial own genome can be removed completely from the viable cell? It would be highly appreciated if someone provide information regarding this query.

04 August 2019 3,012 12 View

How Long cells can survive in cultute medium without FBS?

I am working with Huh7 hepatoma cells. I have to starve the cells for my experimental purposes. If someone would share the experience of cell survival duration without FBS it would be highly...

18 October 2018 649 4 View

How to solve cell fractionation related problem?

Hi everyone, Please help me to solve the problem if you have same experience.After cell fractionation by sonication and centrifugation at 14000 g for 10 minutes ,sometimes I found some cell like...

16 October 2017 5,817 4 View

How long it takes for complete polymerization of SDS-PAGE gel?

How long it takes for complete polymerization of SDS-PAGE resolving (separating) gel? After pouring into the plate it takes one hour for polymerization,is it enough for experiment?

24 May 2016 5,798 18 View

Do the physiology and protein expression level of cancer cells in culture medium change with the number of batches?

If a mutant cell is cultured in a medium for several batches then what happens in case of the physiology and protein expression level?

09 March 2016 2,489 2 View