Md Asaduzzaman

4 Questions 4 Answers 0 Followers

Questions related from Md Asaduzzaman

Small RNA sequencing by HiSeq X platform?

I am now working on small RNA sequencing from exosome. For library preparation I used Takara SMARTer® smRNA-Seq Kit for Illumina®. The sequencing results are not good. The company used HiSeq X ten...

23 June 2020 4,772 3 View

Why HiSeq X ten platform can not be used for small RNA sequencing?

From websites, I understand that HiSeq X ten platform only compatible for whole genome sequencing and it is not compatible for small RNA sequencing? However, HiSeq 2500 is regularly used for small...

23 June 2020 4,195 0 View

How long the morphology of exosome remain intact for TEM analysis?

We have isolated the exosome and preserve it at -20 degree freezer for about one month. Is the sample still suitable for morphology check under transmission electron microscopy or we must need to...

29 May 2019 9,445 5 View

I would like to isolate exosome from blood samples of fish and shrimp for various downstream analysis. Could anyone suggest best protocol?

Please note that the fish size is small, so we will not enough blood for exosome isolation.

21 January 2019 8,346 5 View