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Questions related from Mazhar Hussain
Dear all, suppose I am working on a machine learning problem where I am trying to predict an attacking site of an enzyme on its substrate. example: Let suppose an enzyme attacks on aspartic acid...
08 August 2018 9,344 2 View
Hello everybody, I have been using Hoefer SDS PAGE system. It gives fantastic bands. But the only problem with this system is that it's combs are open which causes it's wells to shrink down. It...
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Hi everyone, I'm currently working on the splicing variants of a protein. For the analysis, I extracted variants from two databases in Fasta format. Before downstream processing, I want to make...
02 February 2016 1,428 11 View
I have been working on a protein which is get cleaved at its c-terminal in response to a viral infection. Whenever, I see my western blots, I can see partial or complete cleavage with two bands....
10 October 2015 5,492 10 View
Hi everyone, I have been working on real-time quite alot. Recently, I performed an experiment with 3 repeats. For me, repeat means doing same experiment again, but at different time. So, I did...
08 August 2015 2,932 5 View
Hello everyone, After trypsin-EDTA treatment to the cells, I resuspend MDCK cells in 10ml of MEM (10%FBS). Then, once I load 10 micro-L of the suspension on Hemocytometer, I get clumps and very...
06 June 2015 9,041 2 View
Hi everyone, Recently, I have been working on effect of a protein on the expression or activity or stability of another protein. But, I know one thing that the protein which is modulating the...
06 June 2015 9,159 3 View
Hi everyone, Currently, I am designing primers for the real-time analysis. I have observed in the nucleoprotein and PB2 of the influenza virus that a huge amount of variation is consistently...
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