Maximiliaan Lens

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Questions related from Maximiliaan Lens

Can MMA embedded undecalcified bone be used for TEM and SEM?

I thought that I had read somewhere that MMA cannot by used for TEM/SEM, but I cannot remember where. I have already found a publication stating that it can be used, but not much information about...

24 June 2015 1,543 5 View

Why does my Goldner stain fail to stain metaphyseal mineralized bone?

I have performed a goldner trichrome stain to perform histomorphometric analysis of the metaphyseal zone of MMA embedded bone. But I fail to stain the mineralized metaphyseal bone, the rest of my...

08 April 2015 8,803 3 View

Where can I find protocols for Bone IHC markers: ALP, TRAP, COMP, collX, CD31 and an apoptotic marker?

We're setting up different protocols for bone IHC. We have already got some good results, but fail to obtain perfect results. So we were wondering if we have done something wrong, a...

02 February 2015 8,149 0 View

What is the dehydration time of rat proximal femur parts for MMA embedding?

I've read several articles and notes and times vary from 1 day per step to 3 days per step. The steps I would like to take are: alcohol 50%, alcohol 75%, alcohol 96% , alcohol 100%, Xylene twice....

16 January 2015 8,036 0 View