Matthew Penney

6 Questions 1 Answers 0 Followers

Questions related from Matthew Penney

EDNA Filter Sub-sectioning and Preservation Methods?

We're looking into ways to subsample filters from eDNA runs (so we don't have to use entire filters all at once) and to preserve filters indefinitely until we need them. Freezing maybe makes...

28 October 2022 8,465 0 View

Getting Consistent Issue With Arlequin Mantel Test: Formatting Issue?

Pardon the file names. I was having what might be called a Bad Day. Anyway, whenever I try to run this file and perform a Mantel Test I get the following error: Error while checking matrices,...

20 June 2022 4,269 0 View

STACKS process_radtags: all reads returned as "ambiguous barcodes"?

Hello, I am running process_radtags 2.53 on a set of sequences obtained from an Illumina NovaSeq 6000 platform at Genome Quebec. They were able to demultiplex the reads already (I'm just doing...

19 April 2021 7,474 0 View

Adegenet function "df2genind" problem: dataframe read as one individual?

Hello, I'm trying to create a genind object of an SNP dataset from Whelk to examine allelic richness between sites for some COI_16S sequences (combined sequences from the same individual). My...

07 October 2020 9,390 1 View

Any RADseq experts who can troubleshoot a protocol?

I am having difficulty developing a protocol for the successful amplification, extraction, and sequencing of ddRAD libraries from sea cucumbers (Cucumaria frondosa). Recently, I sent libraries...

24 August 2020 6,166 3 View

Trouble configuring VCFtools on GitHub using Git Bash, anyone help?

I'm trying to clone the respository for VCFtools into GitHub and configure it. These two scripts always run successfully in GitBash: git clone https://github.com/vcftools/vcftools.git cd...

20 January 2019 7,478 2 View