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Has anybody attempted to isolate RNA from low numbers (~5,000) unstimulated CD8+ lymphocytes? I've attempted using the usual kits (Qiagen RNA/DNA micro; Qiagen RNA micro, Zymo Direct-zol...
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Hello All - I am FACS sorting a rare T-cell subset (~0.1% of all CD8+ T-cells) for later epigenetic (using RRBS) and transcriptomic analysis. I aim to extract DNA/RNA from my target cells using...
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