2 Questions 2 Answers 0 Followers
Questions related from Matt Mee
Has anybody attempted to isolate RNA from low numbers (~5,000) unstimulated CD8+ lymphocytes? I've attempted using the usual kits (Qiagen RNA/DNA micro; Qiagen RNA micro, Zymo Direct-zol...
29 April 2019 6,526 6 View
Hello All - I am FACS sorting a rare T-cell subset (~0.1% of all CD8+ T-cells) for later epigenetic (using RRBS) and transcriptomic analysis. I aim to extract DNA/RNA from my target cells using...
03 October 2018 561 4 View
