Maryam Zare

5 Questions 12 Answers 0 Followers

Questions related from Maryam Zare

TagBFP expression under MOUSE phosphoglycerate kinase promoter?

Hi all, I have transfected LentiX293 T cells with a plasmid expressing TagBFP under mouse PGK promoter. However, after checking under microscope, I can not see any signal. Can having a mouse...

25 April 2023 2,661 4 View

Cas9-loaded VLPs. LentiX 293 T cells or Gesicle-producer 293 T cells?

Hi all, I am trying to make Cas9-loaded VLPs. Is there any difference between using Lenti-X 293 T cells or gesicle-producer 293 T cells? Could it affect VLP titer? What is the main difference...

18 January 2023 6,551 0 View

RNA isolation and cDNA synthesis from low number of cells(human regulatory T cells) ?

Hello I am working on Human regulatory T cells. I culture PBMCs and stimulate them. After that, I isolate subpopulation of regulatory T cells using MACS technique and then perform syber green...

06 October 2016 4,363 3 View

What is the appropriate wavelength for MTT assay?

Hello, I am performing MTT assay on PBMCs. For adjusting cell number, Briefly I seed different number of cells/well, incubate 24 and 48h, add MTT and incubate, finally add DMSO as solvent and read...

24 August 2016 3,963 17 View

Can positive selection strategy of MACS Technique interfere with qRT-PCR and mRNA expression level of molecules?

Hello, I want to culture human PBMCs, Isolating a subpopulation of T cells by means of MACS Isolation technique (Positive Selection Strategy), and then investigating mRNA expression level of some...

24 April 2016 9,868 4 View