Marwa Amer

9 Questions 33 Answers 0 Followers

Questions related from Marwa Amer

CPM assay with rotor gene Q?

Hi, I'm using the rotor gene q for my CPM assay and in the setup of the analysis I have to adjust the ramping rate to be 2˚C/min but the only way to do this is to adjust (wait for -- seconds for...

01 January 2017 8,798 0 View

Can any one help me with data analysis of Dual luciferase experiment?

Hi I did an experiment for miRNA and targets but I have a problem in calculating the significance of the data can you help me which test should I have to use and how to caluclte the p value or z...

06 June 2016 6,349 4 View

Why there's no expression in western Blotting for transient expression?

I'm trying to express membrane protein gene in pACMV-tetO Flag taged one. I did a transient expression in HeK293 Gent- but when I did a western blotting (without purification) only the cell lysate...

06 June 2016 4,777 8 View

Problem in qPCR amplification plot?

I'm doing qPCR reaction using SYBEr green master mix Takara, When I did the reaction for the first time, I've got a good result but when I repeated it I got a strange plot that I can't explain. So...

01 January 2016 6,948 4 View

What's the optimum temperature for saving tissues in formalin or paraffin?

I want to isolate RNA in high quality to be used in RNAseq experiment from Paraffin or formalin. What i want to know are: 1- The optimum temperature for saving tissues in formalin or paraffin 2-...

02 February 2015 8,113 4 View

How I can get RNAseq data-set for hepatocellular carcinoma?

I want to work on different RNAseq data-set for Hepatocellular Carcinoma preferably at different stages of the disease. I searched for these data-sets but I couldn't find them through the cancer...

05 May 2014 5,191 7 View

Is there any common antibiotic that can work on both puromycin and neuomycin resistant genes for the selection of transfected cells?

I want to transfect Huh7 with two different vectors one containing neomycin resistance gene and the other containing puromycing resistance gene. Does one of them affect the other or is there any...

11 November 2012 834 10 View

Any recommendations for starting micro array data analysis?

I'm beginning the analysis of my micro array experiment of me and I don't know which program is the most user friendly. What are the parameters for data analysis?

10 October 2012 2,417 15 View

Double Restriction digestion of a plasmid

I'm cutting miRNA expression vector with BamHI and NheI. I did the BamHI digestion by different conditions then cut with NheI. The problem is I have unexpected bands at some conditions and the...

09 September 2012 8,998 17 View