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Questions related from Martin Ballaschk
For my NMR studies, I need multi-mg quantities of 9-mer isotopically labeled peptides. Instead of expensive solid-phase-synthesis, I use a cheap bacterial expression system which is based on...
02 February 2015 4,993 3 View
I have an ancient plasmid (pHN) which I've been told works beautifully in Xa90 cells, and so far I've not been successful in getting it to work in the strains we use at our facility. How could I...
14 May 2014 1,967 3 View
When measuring R1, R2, and Hetero-NOEs of large proteins, the overall experiment time can easily exceed weeks, especially when measuring at different fields. As always, one has to trade off...
19 March 2014 7,635 16 View
When measuring relaxation rates, we routinely also record Hetero-NOEs of the (H)-N amide bond. In order to do that, we collect reference and saturated spectra in a FID-interleaved manner to...
10 September 2013 8,776 13 View
Does anyone know a bunch of reviews or a guide that covers basic practical questions associated with the establishment of CPMG relaxation dispersion experiments for the study of protein motion?...
25 January 2013 1,673 7 View
I recorded T1 and T2 relaxation data at two different fields (600 and 750 Mhz) for model-free analysis. A prerequisite is to have absolutely the same experimental conditions at any time during the...
18 January 2013 8,378 2 View
