Marianna D'Anca

6 Questions 6 Answers 0 Followers

Questions related from Marianna D'Anca

Does anyone can suggest me a validated protocol to obtain cortical neurons from iPSC?

Hi, I'm searching a validated protocol to obtain cortical neurons from iPSC. The ideal for me is to have the whole protocol to obtain cortical neurons starting from PBMCs with iPSC passage....

20 April 2022 8,377 2 View

Does anyone suggeste me a validated protocol to obtain lymphocytes from whole blood and how I can mantain them in culture?

Dear all, I'm searching a validated protocol to obtain lymphocytes from whole blood to mantain them in culture and obtain from them iPSC. If someone can give me a protocol starting from whole...

06 April 2022 3,325 2 View

Does anyone know a validated protocol to obtain microglia cells from iPSC?

Dear all, I'm searching a validated protocol to obtain microglia cells from iPSC. Thanks

06 April 2022 2,143 1 View

Hello, I want to know if someone ever did a western blot with an expired mops running buffer with precast gels.

Hello, I want to know if someone ever did a western blot with an expired mops running buffer with precast gels. I used it for my last gel and the ladder was faint on the gel and obviously on the...

20 July 2017 2,150 7 View

Does anyone know teh best method to do a western blot to identify the 14.3.3 protein on CSF?

Dear all, does anyone give me teh best protocol to do a western blot to identify the 14.3.3 protein on cerebral fluid spinal? May I use directly the liquor or should I have to prepare the sample...

01 July 2016 6,076 1 View

Does anybody know the best protocol to extract protein from exosomes? Which is the best method to extract exosomes from blood serum?

Extraction protein from exosomes

24 February 2016 1,485 3 View