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Does anyone can advice me a good protocol? in the lab we currently use Chirgwin protocol but most of us struggle with it as we cannot achieve all the time good quality RNA for experiments.. The...
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Has anybody used any reaction kit to perform click chemistry in tissue? I am interested in performing it with isolated acinar cells or isolated hepatocytes. Does anybody has experience with...
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anybody has seen something like that ever??
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Dear all I am desperately trying to show an increase of h3K4 2me in my protein samples after incubation with an LSD1 inhibitor. I am working with AML cell and my results are variable all the time....
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I am trying to label LSD1 using Click chemistry. I would like to show the fluorescence by SDS Page. It worked so fare with pure protein (37ug) even though I had a lot of Background. I would like...
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