Maria Licursi

6 Questions 9 Answers 0 Followers

Questions related from Maria Licursi

Has anyone tried to stain MRC5 cell membrane with Cholera Toxin B (CTB) conjugated with FITC?

I am trying to stain MRC5 cells with CTB-FITC to look at its membrane with confocal microscopy. I would appreciate if someone can share a protocol with me. In my first try I had a very weak and...

30 July 2024 1,985 0 View

How to calculate the TCID50 when the highest CPE is 50% or less?

I am doing TCID50 to measure progeny virus and some treatments protected the cells in a way that the highest CPE observed is 50% or less. How should I do the titre calculation in that case? Thank you!

17 February 2021 9,240 1 View

I am looking for a good GSK3b inhibitor. Any suggestion?

I need to do some in vitro experiments inhibiting GSK3b. Which is a good inhibitor?

03 May 2019 7,033 1 View

Can someone recommend an ELISA kit to measure Prostaglandin E2 in mouse tissue samples?

I would appreciate any suggestion on ELISA kits to measure PGE2 from mouse tissue and if you can comment on sample preparation or you have a protocol that worked for you. Thanks

22 November 2016 8,922 3 View

I want to use CRISPR to knock down a gene in mouse cell lines. Would the human U6 promoter be functional in mouse cells?

04 August 2016 9,837 2 View

Does anyone have a good and cheap protocol to precipitate the AAV present in the supernatant?

I transfected HEK293T cells with the 3 plasmid method using PEI and I purified the AAV present in the cells with iodoxanol gradient after 3 cycles of freeze/thaw. However, I need a protocol to...

01 September 2015 4,044 7 View