Manjunath Siddaramaiah

4 Questions 6 Answers 0 Followers

Questions related from Manjunath Siddaramaiah

Can somebody help me understand why magnetic beads clump during nucleic acid extraction and how to prevent it ?

Hello everyone, we are doing a nucleic acid extraction using magnetic beads from upper respiratory tract swab sample collected in VTM. Some "random" samples (VTM) when mixed with lysis buffer and...

05 October 2021 7,496 4 View

How to achieve complete and faster release of 150nm glod nanoshells from conjugate pad ??

Dear all I am involved in the development of lateral flow assay for covid N-protein detection. I am facing problem in complete release of conjugate from conjugate pad. I am using glass fiber...

05 July 2021 9,227 3 View

Can some one help me with the calculating the ratio of capture Ab, conjugate and analyte concentration required for the standard competitive ELISA ?

we are developing a standard competitive assay for small bio-molecule. we are regularly noticing a hook kind of higher signal for one of the middle calibrator (Calibrator concentration and purity...

12 August 2020 842 1 View

Lateral flow assay- irregular test line dispensing on NC membrane

I am facing the problem in dispensing test and control lines. Dispensing is not uniform and appears as spots in some places. This is may be because of hydrophobic nature of the NC membrane (non...

01 January 1970 7,847 1 View