Manindra Singh

6 Questions 17 Answers 0 Followers

Questions related from Manindra Singh

Why are HUVECs non-responsive to treatments?

Hello everyone, So I am culturing HUVECs (purchased from Lonza) to estimate their activation responses to some growth factors. However, I notice no change in the output, such as cytokine...

10 October 2015 740 9 View

What is the suitable treatment method for HUVECs for studying inflammatory/ angiogenic gene expression?

Hello all, I recently started growing HUVECs to study the angiogenic and inflammatory response wrt certain treatments. I am using EBM-2 complete medium (2% FBS and growth factors including...

01 January 2015 1,090 3 View

When should murine endothelial cell line treatments include serum starvation?

I wish to treat the murine endothelial cell lines with some factors to check the pro-inflammatory cytokine release. I read some papers and found different treatment strategies. Is it important to...

03 March 2014 2,401 2 View

How to eliminate recurrent contamination in endothelial cell cultures?

I am working with MS1 endothelial cell line (mouse), and have noticed a recurrent contamination which appears after day two in culture. I tried again by changing to new media, new trypsin etc. But...

02 February 2014 5,096 4 View

Has anyone treated endothelial cells with free fatty acids/ triglyceides?

I wish to include a fatty acid treatment in my endothelial cell culture. Please suggest what mixtures would be suitable from the point of activation.

02 February 2014 2,471 3 View

How to normalize the cytokine levels to cell number?

I am trying to quantify the cytokine levels using ELISA at different time points such as 12h, 24, 48 & 72h, from mouse endothelial cells upon hormone treatment. The question is regarding the...

12 December 2013 5,498 6 View