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Hi fellow sciency friends, I have a protocol for IF-FISH that says to use RNaseOut (Invitrogen) in my block at 1:100. I'm doing the IF-FISH on coverslips in a 6-well plate and I'm using 2mL block...
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I am running two westerns with the same samples for a FLAG-tagged Cas9 we transfected into HUES cells. I run both gels (4-12% Bis-Tris) in the same gel box, I transfer them (iBlot) at the same...
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