Maisarah Ab Samad

4 Questions 1 Answers 0 Followers

Questions related from Maisarah Ab Samad

Samtools markdup for duplicate removal or Picard?

Hi everyone... I'm new in NGS analysis and I will work on Chip-seq and RNA-seq Im doing some analysis with published datasets I know this question is common in the NGS forums... When I search...

24 June 2020 6,132 2 View

Affordable machine for bioinformatics with minimum specs?

Hi, currently I'm searching for an affordable laptop with minimum specs for bioinformatics. My work will be NGS analysis (ChIP-seq & RNA-seq) I believe that MacBook is the best choice, but I...

02 June 2020 1,999 4 View

Does adding epitope tag in the middle of Homology arm and far from the DSB (~250 bp) is permissible for successful knock-in?

Hi. I want to knock-in 3xFlag-tag at the 3'end of my target gene, upstream of the stop codon. Hence, I will add the STOP codon after the 3xFlag-tag. Besides, I want to introduce...

19 October 2017 676 1 View

How can I troubleshoot when single digestion is not working?

Hi.. My vector is pGEM-T cloning vector (3kb) and my insert size is 1992 kb (~2kb). I use AgeI and KpnI for double digestion. For the competent cells, i use DH5a to clone the plasmid. I did...

13 June 2015 9,433 3 View