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Questions related from Maibam Singh
Hi. In the past I used glass pipettes to do stereotaxic injections and filled them via capillary action and used mineral oil to avoid dead space. Now in the lab we have WPI nanosyringes that are...
03 May 2023 7,450 1 View
Hi. I am having issue with maintaining positive pressure. This was not the case earlier but suddenly it has become the major factor in deciding if the recording will be a success or a failure....
14 August 2020 371 0 View
Hi, I use a EYFP cre reporter mice and do IHC on them to see the expression pattern. Usually, I slice the brain and on the same day start the IHC protocol with blocking and primary antibody...
16 October 2018 6,789 1 View
TH-GFP (Tyrsosine hydroxylase GFP) is commonly used for identification of dopaminergic neurons in culture. I also came to know about PITX3-GFP mouse that is also gaining popularity for the...
14 December 2017 3,451 0 View
Hello fellow Patchers and Sealers, I made a pipette solution recently and expected the osmolarity to be around 270 mOsm approximately according to rough calculation...
11 August 2017 9,045 2 View
I have had consistent contamination in my primary culture of mouse dopaminergic neurons and I am not able to culture the cells beyond 5 days. From visual inspection, I believe its a fungal...
14 August 2015 4,941 6 View
Hi, Please take a look at the restriction digest blot I have attached here. I ran the reaction twice with changing the parameters a little but still the same result! Here's what I did: Enzymes...
29 April 2015 4,404 6 View
