Maibam Singh

9 Questions 12 Answers 0 Followers

Questions related from Maibam Singh

How to fill WPI nanosyringes (or hamilton syringes) for AAV injections for small volumes ~ 2uL?

Hi. In the past I used glass pipettes to do stereotaxic injections and filled them via capillary action and used mineral oil to avoid dead space. Now in the lab we have WPI nanosyringes that are...

03 May 2023 7,478 1 View

How to maintain positive pressure or solve the problem with suction?

Hi. I am having issue with maintaining positive pressure. This was not the case earlier but suddenly it has become the major factor in deciding if the recording will be a success or a failure....

14 August 2020 413 0 View

Is it possible to loose nascent fluorescence (EYFP) in slices due to longer storage?

Hi, I use a EYFP cre reporter mice and do IHC on them to see the expression pattern. Usually, I slice the brain and on the same day start the IHC protocol with blocking and primary antibody...

16 October 2018 6,817 1 View

What is the best software for analyzing spontaneous action potentials and spontaneous currents?

Hi, I am looking for a software that can analyze recordings of spontaneous action potentials and spontaneous currents. Not just limited to it but can also be used to analyze other recordings...

03 March 2018 9,369 7 View

A transgenic mouse model for identifying dopaminergic neurons in the midbrain?

TH-GFP (Tyrsosine hydroxylase GFP) is commonly used for identification of dopaminergic neurons in culture. I also came to know about PITX3-GFP mouse that is also gaining popularity for the...

14 December 2017 3,479 0 View

Manual osmolarity calculation for internal patch solution!

Hello fellow Patchers and Sealers, I made a pipette solution recently and expected the osmolarity to be around 270 mOsm approximately according to rough calculation...

11 August 2017 9,128 2 View

Glial/astrocyte growth inhibitor for hippocampal and midbrain dopaminergic culture?

Hi, In our lab we combine whole cell electrophysiology and TIRF microscopy based calcium imaging to measure transient calcium influx through calcium channels present in the membranes. I use...

20 June 2016 9,732 8 View

Why do i get contamination with primary culture of dopaminergic neurons from mouse brain?

I have had consistent contamination in my primary culture of mouse dopaminergic neurons and I am not able to culture the cells beyond 5 days. From visual inspection, I believe its a fungal...

14 August 2015 4,980 6 View

Is the problem with the DNA sample or did the restriction digestion not go well?

Hi, Please take a look at the restriction digest blot I have attached here. I ran the reaction twice with changing the parameters a little but still the same result! Here's what I did: Enzymes...

29 April 2015 4,434 6 View