Mahsa Khayat-Khoei

4 Questions 3 Answers 0 Followers

Questions related from Mahsa Khayat-Khoei

How to passage and maintain healthy human astrocytes?

Hi all, I have bought a line of human glia (astrocytes) and want co-culture it with cortical neurons differentiated from iPSCs. I want to maintain these cells and passage them for future use, I...

21 August 2021 3,814 0 View

How to cryopreserve healthy human astrocytes?

I have a line of healthy human astrocytes, and plan to passage it. I was wondering if any suggestions on how to cryopreserve it? I use mFreSR from stem cell to freeze the iPSCs and not sure if I...

21 August 2021 5,372 4 View

Has anyone ever kept the whole Matrigel bottle in 4C instead of making aliquots?

Hi all, Here is a simple technical question: I have been making aliquots of Matrigel and saving them for each experiment. However, an expert in our lab, who has now left, showed me a few weeks...

04 May 2021 3,681 0 View

Black dots and round shaped structures formed on iPSC culture, is it contamination?

Hi! I have been passaging iPSCs in StemFlex no antibiotics, and in the most recent culture I noticed many black dots and particles only on a few parts of the well and not through the whole...

11 March 2021 6,298 6 View