Magnus Olsson

6 Questions 6 Answers 0 Followers

Questions related from Magnus Olsson

Is there a variation in CMV expression in between individual cells after lentiviral transductions?

In line with previous data suggesting low efficacy of the CMV promoter in some cell lines (Qin et al. PlosOne 2010), I experience low transcriptional activity in a neuroblastoma cell line after...

11 February 2020 2,374 3 View

Can anyone suggest a reliable software/search engine that relates a gene product to defined molecular pathways?

The particular question I have is the following: Starting with three separate proteins, is there a bioinformatic tool which can be used to identify one or several molecular signaling pathway in...

13 April 2016 389 4 View

Is there a method for quantification of lysosomal exocytosis in cultured cells?

Lysosomes are primarily involved in degradation and recycling processes. For still unknown reasons they are apparently also releasing their content into the extracellular space by means of a...

14 December 2015 9,901 4 View

What is the best way to verify that cells die by excess autophagy?

There are robust techniques for the analysis of apoptotic and necrotic cell death modes. However, what methodology can be used to specify autophagic cell death and to discriminate this particular...

26 March 2014 6,176 6 View

Which antibody directed to human 53BP1 can be used in western blot and immunocytochemistry?

Is there someone that can recommend a reasonably priced anti-human 53BP1 antibody which can be used both in western blot analysis and in immunocytochemistry?

14 January 2014 5,797 1 View

Can detection of Poly(ADP-ribose) (PAR) polymers be used as an indicator of DNA stress?

Poly(ADP-ribose) polymerase-1 (PARP-1) is a nuclear protein that is involved in the DNA base excision repair system (BER), where it is activated by DNA strand nicks and breaks. Using NAD+ as a...

13 December 2013 367 11 View