4 Questions 2 Answers 0 Followers
Questions related from Maciej Florczyk
I’m in the process of developing stable Huh-7 cell line for luciferase purposes. My experiment is mostly based on Connelly’s (2012) work, except I’m using pmirGLO vector instead of psiCHECK-2. Is...
07 July 2018 7,636 3 View
How many base pairs U6 snRNA should have? I'm currently doing northern blot both on fish liver and HEK293 RNA. As a control I'm using U6. From what I've red U6 should be placed around ~120bp, but...
10 October 2017 3,935 0 View
I'm looking for reliable zebrafish ago2 (eif2c2) antibody for IP. I found one form abcam (http://www.abcam.com/ago2--eif2c2-antibody-chip-grade-ab57113.html) (any experiences?) but its value/price...
04 April 2016 3,806 0 View
The image shows DNA fragmentation assay of cells treated with toxin. I'm not sure how to read those results. For sure there is fragmentation in well with number 1, but what about wells 2 and 3?...
10 October 2015 4,152 5 View
