Lydia Horndler

4 Questions 4 Answers 0 Followers

Questions related from Lydia Horndler

What is the best protocol to fix cell in order to visualize the membrane by Scan electron microscopy (SEM)?

Hi, I want to study the membrane using SEM, I am having some problems with cell fixation. I am using PFA and glutaraldehyde , and one lasts step with HDMS ( hexamethyldisilazane). But I think the...

22 November 2019 609 3 View

Persister cell of S epidermidis

I need a protocol to generate persistent cells of S. epidermidis because I want to test my agent only against to persiter cells of s epidermidis. I think maybe I could growth a biofilm and treat...

13 July 2017 941 3 View

Develop a washing machine conditions to growth a biofilm?

I would like to do a test with the most similar washing machine conditions in order to evaluate a surface developed to prevent biofilm growth. i would like to do in a short time if it was...

18 May 2017 332 3 View

I am trying to do a MIC minimal inhibitory concentration,my solut isnt soluble,is like a gel and it is precipited by water, somebody know how to make?

i need to know how to do a mic whit no soluble agent, I looked about quartenarium amonium MIC but my big problem is that my solut is a gel and i cant to control contact surface if i made a coating...

26 October 2016 7,442 3 View