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Other than using a positive control, is there a way to determine if the Genscript anti-his elisa protein quantification kit underestimated the protein concentration? Because the discrepancy using...
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I could not really find a protocol specifically stating how to induce protein expression under AOX1 promoter in Pichia pastoris. Do we straight away streak them out on BMMY agar? Or streak them...
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I am trying to create a library of promoters to test them on my enzymes. I bought two kits from addgene which contained the promoters. However, for the same Promoter, i realized one has the kozak...
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I have a mixture of plasmids (with Origin of replication that can work in Pichia pastoris) with different genes for protein expression. Is it possible that after chemical heat shock...
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I frequently need to do plasmid preparation and maxiprep does not work so we stick with miniprep. For our bacteria stock, we kept it in glycerol and usually inoculate them straight from the...
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I did a BrdU assay on the liver cells (L02) and the incubation time (with BrdU) was 24 hours. However, not much cells had the BrdU incorporated. Also, I do not have positive control. How do I...
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