Lutz Grohmann

3 Questions 8 Answers 0 Followers

Questions related from Lutz Grohmann

Is it effect of single mismatch in TaqMan probe binding?

Hello, after validation of a TaqMan PCR assay we idenditifed one single mismatch to a group of target sequences. The mismatch is located at the first base at the 5' end of the probe. See (mismatch...

21 January 2016 4,165 11 View

Why is it better to linearise a control plasmid before PCR?

Control plasmids carrying a specific target sequence are very useful in PCR analyses, e.g. as positive control or as calibrant. Nowadays, customised plasmids can be easily purchased from service...

24 August 2015 6,136 7 View

Which methods are capable for the detection and identification of genome-edited (crispr/cas etc) crops?

I am currently thinking about the difficulty of detecting and identifying the genome-edited (crispr/cas mediated) crops. If the changes are only few nucleotides I think they will be...

01 January 1970 1,616 4 View