Long Pham

10 Questions 24 Answers 0 Followers

Questions related from Long Pham

How to develop a 0.5-um thin SU-8 layer?

Hi, I tried to generate an array of thin line (0.75-um wide) and 2-um spacing using 1-um thick SU-8 layer. - The wafer was precleaned with acetone, Isopropanol, and water and heated at 180 C...

15 July 2018 8,707 8 View

Waste products of fibroblasts?

Hi all, During the in vitro metabolism of fibroblasts, what kind of waste this cell generate? We are trying to explain how these wastes may affect the cell migration. Thanks a lot for your...

03 November 2017 2,524 1 View

What is the best fluorescent marker/cell line/animal for Live imaging of actin filament in mesenchymal stem cells over long time (2wks)?

We want to image actin activity of mesenchymal stem cells (MSCs) over a period of 2 weeks without sacrificing them. We want MSCs to be stably expressing the fluorescent proteins at the actin...

02 December 2015 3,003 4 View

Which pipettors should I choose?

I am trying to buy some micropipettors for my biomedical lab. I try to dispense liquid in quite a large range from 0.1 uL to 1000 uL. But I am confused about which one to choose. Some are very...

09 September 2015 5,296 10 View

Can I buy cheap SU8 somewhere?

I want to run some soft-lithography treatment and looking for a photoresist. SU8 seems to be very good but the price offered by MicroChem is so expensive (upto > $500/ 500ml bottle). Can we buy...

17 August 2015 4,707 3 View

What companies can print photomasks to be used with a mask aligner in US?

Hello, I just have a new UV lamp and trying to make some soft-lithography. However, I don't know which companies are printing photomasks in US. I wonder if somebody can give me some contacts? The...

17 August 2015 3,399 8 View

How can I store rats organs for future (stem) cell extraction?

I currently want to dissect a rat and store its organs inside liquid nitrogen for future cells or stem cell extraction, including heart, liver, kidney, bone marrow, tendon, cardiomuscle, etc. I...

04 January 2015 2,001 8 View

Do we still need FBS and why in cryogenic preservation?

Hi everybody, I have quite a awkward question about the cryogenic storing of cells. I am working on MSCs and I wonder if cells can be stored in LN2 just using normal culture media. People are...

30 December 2014 8,681 9 View

Mesenchymal stem cell isolation from rats: what are the differences between old and young rats?

I am doing the mesenchymal stem cell isolation from rats. I wonder whether there are differences between cells extracted from neonatal rats and from those from older rats. First of all, I found...

25 December 2014 1,337 15 View

How does centrifugal speed affects cells' viability?

I am trying to freeze the bone marrow extract from rats. In a reference protocol, the centrifugal speed is about 450 g in 5 min. But my lab only has a centrifuge with a fixed speed of 980 g. I...

06 November 2014 1,347 10 View