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Questions related from Liyana Azmi
I need a protocol to shear flagella off E.coli O157:H7. I have been using a protocol online that is basically resuspending an overnight culture in a syringe and then, spinning it down to acquire...
30 March 2017 573 2 View
I have a protein which I know is stable with the presence of co-factors (NAD+ and Fe2+). Recently i have been trying to purify it with the TCEP as a reducing agent since my protein is known to...
23 November 2016 7,552 2 View
I amrunning DMD modelling to model possible conformations of my proteins. However when I submit my proteins for DMD modelling, it came back to me reporting that it had steric clashes.. How do I...
17 March 2016 2,780 2 View
I am purifying AdhE (96 kDa) protein which is bifunctional and ontains 2 functional domains; (acetaldehyde dehydrogenase and Alcohol dehydrogenase). Before purification, I did a small scale...
28 August 2015 4,400 6 View
I am doing Chip-seq, but at the end of the experiment I have obtained a clear DNA background for my negative samples but absolutely no DNA from my tested samples. I think there might be something...
04 August 2014 5,480 7 View
I have a protein sequence which I want to see in 3D. I tried Pymol and Cn3D, but the protein files that they have don't have the sequence which I want to see. I have to predict the protein...
18 March 2014 8,835 21 View
I made ampicillin plates (1:1000 ampicillin dilution that is 400ul in 4 mls of LB broth) and grew my transformants (dh5 alpha + vector pT7-FLAG2 + DNA inserts)into the agar overnight at 37 celsius...
10 March 2014 2,124 10 View
My vector is pT7 FLAG2 sized 4.8kbp and my insert is roughly 2-3kbp. I grew them in BL21 and sub-cultured them overnight with ampicilin before extracting them. After that I digested them with my...
25 February 2014 3,283 14 View
I did my birnboim and doly prep and received multiple bands everytime. I compared the bands with samples using Qiagen miniprep kits and still I have multiple clean bands. When I added RNase to my...
21 February 2014 947 3 View
I'm screening for my plasmids which are pT7-FLAG2 with inserts approximately 1000-3000 bp each. My plasmid is 4.8kbp. I've cultured them in BL21 and tried extracting them using Birnboim and Doly...
20 February 2014 3,930 2 View
I did a Birnboim and Boly plasmid Prep for my plasmid and insert which are grown on BL21. After that, I digested them with my restriction enzymes and run the samples on the gel. What I got was a...
19 February 2014 3,903 3 View
Specifically, I'm looking for anti-FliD (also known as HAP2) for Escherichia coli O157:H7, the capping protein at the end of the filament.
01 January 1970 2,232 2 View
I'm purifying a protein that has a functional cysteine residue at the active site. In the file below, on the left is the SDS of the protein post-IMAC purification. These fractions are then...
01 January 1970 1,797 3 View
Roche provides an intereactive complete metabolic pathway map online however, I can't seem to be able to search for the metabolie/enzyme/anything on the map using the options provided. (here's the...
01 January 1970 694 2 View
