Liviu Iancu

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Questions related from Liviu Iancu

Does anyone have any tips for differentiating P19 cells into cardiomyocytes using DMSO and suspended cell aggregate formation?

This is a general outline of the procedure we've used: 1. Suspended 600,000 cells in 6 mL of alpha-MEM supplemented with ~1% DMSO, 10%FBS, and 1% Pen/Strp (antiobiotic). 2. Cultured them for 4...

10 July 2015 6,200 3 View

Is there anyway to stain for intracellular cardiomyocyte markers (like cardiac troponin) without fixing or permeabilizing the cell monolayer?

I understand some nonpolar dyes like Draq5 are able to travel through the cell membrane and into the nucleus. Are there any such dyes that stain for any of your stereotypical cardiac markers like...

15 May 2015 5,376 1 View