4 Questions 6 Answers 0 Followers
Questions related from Linn Amanda Syding
Hello there, I am culturing cortical neurons from E17 mice and will need to transduce them on DIV7. Does anyone have a protocol on how to do so best? Any ideas on MOI? Im using CRISPRa plasmids,...
11 December 2020 7,991 2 View
Hi, I am trying to blot a protein of 8kDa with western blotting. So far I have tried to use 0.45um & 0.2um nitrocellulose membranes for transfer as well as 0.2um PVDF. I have tried to use...
02 October 2019 7,806 5 View
Dear reader, I am searching for a protocol on isolation and enrichment of spermatogonial stem cells (SSCs) that is suitable for C57BL/6 mice without a mixed background. As far as I have been...
05 September 2018 2,618 3 View
Hello! Last week I found myself researching how different storage of cells in RLT buffer might affect the end results and found many different answers. Some say it is best to snap freeze the cells...
01 January 1970 2,465 6 View
