Lindsay Fulton

4 Questions 6 Answers 0 Followers

Questions related from Lindsay Fulton

Can I use touchdown PCR for a 16S microbiome sequencing study?

Hi everyone. Just looking for some thoughts on this. My thinking is that I shouldn't use touchdown PCR to amplify 16S regions in this case because it will cause bias. The primers will already...

01 January 2020 8,034 1 View

Why are my P values higher when I include my blocking structure?

Hi all, I am analysing body weights and feed intakes from a study involving chickens. I had 4 treatments in a randomised block design, 20 pens per treatment. I arranged the treatment groups into...

09 September 2019 2,732 5 View

PCR unwanted faint bands. Photo included! Any advice, please?

Hi everyone, does anybody have any ideas for things I can try to get rid of this non-specific amplification? These products are for Illumina sequencing - I used barcoded V4 16S primers below: F:...

09 September 2017 3,315 0 View

Has anybody used Q5® Hot Start High-Fidelity DNA Polymerase?

Hi guys, I'm looking for a polymerase for PCR to construct libraries for sequencing. I have 790 samples. I've been told by the sequencing company that I need a high-fidelity polymerase and they...

05 May 2016 7,475 8 View