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Questions related from Leroy Versteeg
In order to measure cytotoxic activity of T cells, we are trying to set up the DELFIA Cell Cytotoxicity assay. After many different attemps, we are still dealing with a high degree of spontaneous...
10 November 2022 4,388 0 View
Hi! I would like to analyze which peptides are presented on the MHC class I of a parasite-infected cell line. After I infect the adherent cells I need to remove them from the culture flask and...
23 June 2020 6,286 0 View
Hi, I am planning to isolate the MHC-peptide complexes from mouse MC57G fibroblasts which express H-2Kb and H-2Db. I am planning to order a hybridoma cell line from ATCC which produces...
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Hi all, Recently I have been working on analyzing the immune synapse formation using imaging flow cytometry. I co-culture mouse APCs and T cells and subsequently perform fixation/permeabilization...
05 April 2018 7,999 2 View
I am trying to optimize my flow experiments by titrating my antibodies down. I found the formula to calculate the Separation Index (SI) in this article...
12 December 2015 8,984 5 View
I am looking into the plates that are optimal to re-stimulate murine splenocytes in, and subsequently use the same plate to perform ICS in. There are non-tissue culture treated plates...
14 August 2015 9,619 4 View
Hi all, does somebody knows a publication in which is explained at what time point certain cytokines are being produced by splenocytes (BALB/c) when re-stimulated with antigen? We are doing...
23 June 2015 380 5 View
