Leroy Versteeg

3 Questions 5 Answers 0 Followers

Questions related from Leroy Versteeg

High spontaneous release DELFIA Cell Cytotoxicity assay?

In order to measure cytotoxic activity of T cells, we are trying to set up the DELFIA Cell Cytotoxicity assay. After many different attemps, we are still dealing with a high degree of spontaneous...

10 November 2022 4,273 0 View

How well can an established immune synapse withstand mechanical external forces?

Hi all, Recently I have been working on analyzing the immune synapse formation using imaging flow cytometry. I co-culture mouse APCs and T cells and subsequently perform fixation/permeabilization...

05 April 2018 7,916 2 View

Are non-tissue culture treated plates the ones to use when re-stimulating mouse splenocytes for Intracellular Cytokine Staining (ICS)?

I am looking into the plates that are optimal to re-stimulate murine splenocytes in, and subsequently use the same plate to perform ICS in. There are non-tissue culture treated plates...

14 August 2015 9,545 4 View