Leroy Versteeg

5 Questions 9 Answers 0 Followers

Questions related from Leroy Versteeg

High spontaneous release DELFIA Cell Cytotoxicity assay?

In order to measure cytotoxic activity of T cells, we are trying to set up the DELFIA Cell Cytotoxicity assay. After many different attemps, we are still dealing with a high degree of spontaneous...

10 November 2022 4,313 0 View

How well can an established immune synapse withstand mechanical external forces?

Hi all, Recently I have been working on analyzing the immune synapse formation using imaging flow cytometry. I co-culture mouse APCs and T cells and subsequently perform fixation/permeabilization...

05 April 2018 7,930 2 View

How can I calculate the separation index for a marker in flow cytometry?

I am trying to optimize my flow experiments by titrating my antibodies down. I found the formula to calculate the Separation Index (SI) in this article...

12 December 2015 8,924 5 View

Are non-tissue culture treated plates the ones to use when re-stimulating mouse splenocytes for Intracellular Cytokine Staining (ICS)?

I am looking into the plates that are optimal to re-stimulate murine splenocytes in, and subsequently use the same plate to perform ICS in. There are non-tissue culture treated plates...

14 August 2015 9,556 4 View

After how much time are certain cytokines produced by BALB/c splenocytes when re-stimulated with antigen?

Hi all, does somebody knows a publication in which is explained at what time point certain cytokines are being produced by splenocytes (BALB/c) when re-stimulated with antigen? We are doing...

23 June 2015 335 5 View